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densitometry  (Bio-Rad)


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    Structured Review

    Bio-Rad densitometry
    Densitometry, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 36188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/densitometry+(image+lab/Image+Lab+Software/pmc13181540-322-24-28
    Average 99 stars, based on 36188 article reviews
    densitometry - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Effects of dietary phosphorus and myo -inositol supplementation on NaPi-IIb and TRPV6 protein expression in duodenal apical membranes of laying hens from two strains
    Article Snippet: Detection was performed using 1:2,000 anti-rabbit HRP-conjugated secondary antibodies (7074P2, Cell Signaling Technology, Danvers, MA) and chemiluminescent substrate (WesternBright TM Quantum, Biozym Scientific GmbH, Germany) and imaged with the ChemiDoc TM Touch Imaging System (BioRad laboratories GmbH, Munich, Germany). .. Protein expression was semiquantified by densitometry (ImageLab Software, version 6.0; BioRad laboratories GmbH). ..

    Article Title: Acute Intermittent Hypoxia Exerts Beneficial Effects and Promotes Repair in Male Mice in the Experimental Autoimmune Encephalomyelitis Model of Multiple Sclerosis
    Article Snippet: .. The density of each band on immunoblots was estimated using densitometry in Bio‐Rad Image Lab 6.0.1 Software (Standard Edition) to measure relative differences within protein expression across treatment groups. ..

    Software:

    Article Title: Effects of dietary phosphorus and myo -inositol supplementation on NaPi-IIb and TRPV6 protein expression in duodenal apical membranes of laying hens from two strains
    Article Snippet: Detection was performed using 1:2,000 anti-rabbit HRP-conjugated secondary antibodies (7074P2, Cell Signaling Technology, Danvers, MA) and chemiluminescent substrate (WesternBright TM Quantum, Biozym Scientific GmbH, Germany) and imaged with the ChemiDoc TM Touch Imaging System (BioRad laboratories GmbH, Munich, Germany). .. Protein expression was semiquantified by densitometry (ImageLab Software, version 6.0; BioRad laboratories GmbH). ..

    Article Title: Suppression of CSF2RA macrophage polarisation impacts pathological cardiac remodelling in mice
    Article Snippet: Enhanced chemiluminescent HRP-substrate (catalogue number WBLUF0500, Merck Millipore) was used and protein detection was performed with a Chemidoc MP Imaging System (Bio-Rad). .. Relative densitometry was performed with Image Lab Software, version 6.0.1 (Bio-Rad). ..

    Article Title: Robust Bioconjugated Antigens Induce Immune Responses Preventing Malaria Infection and its Transmission
    Article Snippet: .. The concentration of the antigen and the percentage of the fusion protein relative to the total protein in the particle fraction were determined by densitometry (Image Lab Software, Bio‐Rad Laboratories, USA), comparing the protein band intensities to a standard curve generated from known concentrations of bovine serum albumin (BSA; 62.5–500 ng), as previously described [ ]. ..

    Article Title: Suppression of CSF2RA macrophage polarisation impacts pathological cardiac remodelling in mice.
    Article Snippet: Enhanced chemiluminescent HRP-substrate 689 (catalogue number WBLUF0500, Merck Millipore) was used and protein detection was 690 performed with a Chemidoc MP Imaging System (Bio-Rad). .. Relative densitometry was 691 performed with Image Lab Software, version 6.0.1 (Bio-Rad). ..

    Article Title: Histone methyltransferase PRDM9 promotes survival of drug-tolerant persister cells in glioblastoma
    Article Snippet: Detection was performed with Immobilon Western HRP Substrate Luminol-Peroxidase kit (MerckMillipore, Cat # WBKLS0500) and the ChemiDoc MP Imaging System (Bio-Rad). .. Densitometry quantification was done in ImageLab software (Bio-Rad). .. The following antibodies were purchased from Cell Signalling Technologies: GAPDH (1:2000, Cat# 97166), H3K4me1 (1:1000, Cat# 5326), H3K4me2 (1:1000, Cat# 9725), H3K4me3 (1:1000, Cat# 9751S), H3K27me1 (1:1000, Cat# 84932), H3K27me3 (1:1000, Cat# 9733), Histone H3 (1:2000, Cat# 4499), Cleaved Histone H3 (Thr22) (1:1000, Cat# 12576), DHCR24 (1:1000, Cat# 2033), HMGCS1 (1:1000, Cat# 36877S), SETD1B (1:1000, Cat# 44922S), Rabbit IgG HRP-linked (1:2000, Cat# 7074) and Mouse IgG HRP-linked (1:2000, Cat# 7076).

    Article Title: Celastrol overcomes 5-fluorouracil resistance in osteosarcoma cells through p53-mediated apoptotic pathway modulation and P-glycoprotein inhibition: A comprehensive mechanistic study
    Article Snippet: Secondary HRP-linked antibodies (1:3000; Cell Signaling Technology, Danvers, MA, USA) were applied for 1 h. Bands were detected with SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher Scientific) on a ChemiDoc imaging system (Bio-Rad). .. Densitometry used Image Lab software (Bio-Rad), with β-actin for normalization. ..

    Article Title: Histone methyltransferase PRDM9 promotes survival of drug-tolerant persister cells in glioblastoma.
    Article Snippet: Detection was performed with Immobilon Western HRP Substrate LuminolPeroxidase kit (MerckMillipore, Cat # WBKLS0500) and the ChemiDoc MP Imaging System (Bio-Rad). .. Densitometry quantification was done in ImageLab software (Bio-Rad). .. The following antibodies were purchased from Cell Signalling Technologies: GAPDH (1:2000, Cat# 97166), H3K4me1 (1:1000, Cat# 5326), H3K4me2 (1:1000, Cat# 9725), H3K4me3 (1:1000, Cat# 9751S), H3K27me1 (1:1000, Cat# 84932), H3K27me3 (1:1000, Cat# 9733), Histone H3 (1:2000, Cat# 4499), Cleaved Histone H3 (Thr22) (1:1000, Cat# 12576), DHCR24 (1:1000, Cat# 2033), HMGCS1 (1:1000, Cat# 36877S), SETD1B (1:1000, Cat# 44922S), Rabbit IgG HRP-linked (1:2000, Cat# 7074) and Mouse IgG HRP-linked (1:2000, Cat# 7076).

    Article Title: Acute Intermittent Hypoxia Exerts Beneficial Effects and Promotes Repair in Male Mice in the Experimental Autoimmune Encephalomyelitis Model of Multiple Sclerosis
    Article Snippet: .. The density of each band on immunoblots was estimated using densitometry in Bio‐Rad Image Lab 6.0.1 Software (Standard Edition) to measure relative differences within protein expression across treatment groups. ..

    Concentration Assay:

    Article Title: Robust Bioconjugated Antigens Induce Immune Responses Preventing Malaria Infection and its Transmission
    Article Snippet: .. The concentration of the antigen and the percentage of the fusion protein relative to the total protein in the particle fraction were determined by densitometry (Image Lab Software, Bio‐Rad Laboratories, USA), comparing the protein band intensities to a standard curve generated from known concentrations of bovine serum albumin (BSA; 62.5–500 ng), as previously described [ ]. ..

    Generated:

    Article Title: Robust Bioconjugated Antigens Induce Immune Responses Preventing Malaria Infection and its Transmission
    Article Snippet: .. The concentration of the antigen and the percentage of the fusion protein relative to the total protein in the particle fraction were determined by densitometry (Image Lab Software, Bio‐Rad Laboratories, USA), comparing the protein band intensities to a standard curve generated from known concentrations of bovine serum albumin (BSA; 62.5–500 ng), as previously described [ ]. ..

    Western Blot:

    Article Title: Acute Intermittent Hypoxia Exerts Beneficial Effects and Promotes Repair in Male Mice in the Experimental Autoimmune Encephalomyelitis Model of Multiple Sclerosis
    Article Snippet: .. The density of each band on immunoblots was estimated using densitometry in Bio‐Rad Image Lab 6.0.1 Software (Standard Edition) to measure relative differences within protein expression across treatment groups. ..



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    Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and <t>densitometry</t> analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.
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    Bio-Rad densitometry results
    Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and <t>densitometry</t> analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.
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    Image Search Results


    Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: Simultaneous loss of ciliary ARL13B and PC1 rescues fibrosis and injury caused by loss of PC1 alone, as indicated by morphological ( a ), molecular ( b, c ), and cellular ( d, e ) analysis. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-smooth muscle actin (α-SMA) in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 5-6). Scale bars are 200 µm (a) and 50 µm (e). Genotypes in b-e correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Staining, Western Blot, Marker, Control

    ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: ( a ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. (b, c) Densitometry analysis of ( b ) β-catenin and ( c ) cyclin D1 ( n = 3). Genotypes in b, c correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Western Blot, Control

    Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Journal: bioRxiv

    Article Title: Ciliary ARLs drive renal cystogenesis

    doi: 10.1101/2025.11.20.689124

    Figure Lengend Snippet: Morphological, molecular, and cellular analysis reveal that ARL13B R79Q rescues fibrosis, injury, and Wnt signaling caused by loss of PC1. ( a ) Sirius red and fast green staining reveals fibrosis in cyst-adjacent areas. ( b, c ) Western blot and densitometry analysis of the myofibroblast marker α-SMA in kidney lysates ( n = 3). ( d, e ) Quantification and images of renal injury marker SOX9 in kidney sections ( n = 6). ( f ) Western blot of Wnt signaling components β-catenin and cyclin D1 in kidney lysates. ( g , h ) Densitometry analysis of ( g ) β-catenin and ( h ) cyclin D1 ( n = 3). Scale bars are 200 µm ( a ) and 50 µm ( e ). Genotypes in b-h correspond to symbol colors and shapes indicated in a . Data presented as mean ± SEM. Statistical analysis is one-way ANOVA with Tukey’s multiple comparisons test. Asterisk ( * ) indicates significant difference (p < 0.05) from control; dagger ( † ) indicates significant difference (p < 0.05) from Pkd1 fl/fl ; PTCre single mutants.

    Article Snippet: Densitometry analysis was performed with ImageLab software version 6.1 (Bio-Rad).

    Techniques: Staining, Western Blot, Marker, Control